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文献和实验require compensation controls where the same cells are stained with each of the fluorochromes separately. The correct negative control for allimmuno fluorescent experiments are cells treated in the same way but incubated with isotype matched control
Immunohistochemisty-Fluorescence Protocol
to the cells. Isotype matched control immunoglobulin: Stain the cells by a primary isotype pre-matched immunoglobulin of irrelevant antigen-specificity at the same concentration as the cytokine-specific antibodies. Unlabeled antibody control: Cells
Immunohistochemistry: Fluorescence Protocol 2.0
of the cytokine-specific antibody with its target cytokine at a molar ratio of 1:10 prior to addition to the cells. Isotype matched control immunoglobulin : Stain the cells by a primary isotype pre-matched immunoglobulin of irrelevant antigen-specificity
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