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for 1 hour with a 1 :1000 dilution of rat IgG2 a anti-mouse kappa chain monoclonal antibody conjugated to horse-radish peroxidase (LO-MK1 , LO/IMEX) reveal with o-phenylenediamine dihydrochloride (OPD) in revelation solution. SOLUTIONS
-purified goat anti-mouse IgG (Jackson Labs; 1 mg/ml stock used at 1:300 dilution) Texas Red-conjugated affinity-purified donkey anti-rabbit (Jackson Labs;1 mg/ml stock used at 1:200 dilution)
Measurement of HAMA and Anti-Idiotypic Antibodies
). Even in the absence of such immunization some patients may possess HAMA activity because of polyclonal IgM rheumatoid factors that crossreact with Fc epitopes on both human and mouse IgG (1 ).
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