Goat anti-Mouse IgG (H&L) - Af
finity Pure, DyLight®650 Conjugate, min x w/bovine, goat, human, rabbit, rat IgG (highly absorbed against rat IgG)- ￥5520
- Cedarlane
- CLCC32018
- 2025年07月16日
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- 库存：
999
- 规格：
1 mg
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文献和实验E L I S A w i t h p l a t e l e t s
for 1 hour with a 1 :1000 dilution of rat IgG2 a anti-mouse kappa chain monoclonal antibody conjugated to horse-radish peroxidase (LO-MK1 , LO/IMEX) reveal with o-phenylenediamine dihydrochloride (OPD) in revelation solution. SOLUTIONS
, incubate for 30 min at 37°C. ( 5g powdered milk in 95 ml PBS)5 Rinse with PBS 3x.6 Add 50 µl of antibody, incubate 30 min at 37°C.7 Rinse with PBS 3x.8 Add 50 µl of goat anti-mouse IgG conjugate with alkaline phosphatase.(1 µl of conjugate in 4 ml of PBS
(adjust pH to 7.8 with Binding buffer; red color) to the Protein A column.Mouse antibodies of the IgG1 subclass do not have a high affinity for protein A. Purification on protein A beads using standard conditions will yield approximately 1/10
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