Goat anti-Human IgM (µ chain),
- Affinity Pure, Biotin Conjugate, min x w/human IgA + IgG- ￥1760
- Cedarlane
- CLCC21015
- 2025年07月10日
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- 库存：
999
- 规格：
1.0 mg
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文献和实验Mapping Protein Distributions on Polytene Chromosomes by Immunostaining
. x. Place 50 µL of the biotin-avidin-HRP mix on the preparation and cover with a coverslip. Incubate the slides for 40 min at room temperature in a humid chamber. xi. Wash slides in wash solution 1 for 15 min
5- add the AP conjugated tertiary antibody (50 to 100 µl) and incubate for 15 min. The tertiary antibody should be absorbed against human serum; if not add 1% human serum before use. SBA Goat anti mouse AP or Goat anti Rabbit AP can be used
Immunohistochemisty-Fluorescence Protocol
: If using R&D Systems biotinylated antibody skip steps 9 and 10 and continue. Incubate each cell spot on slides for 30 minutes at room temperature with 15 µL of a biotinylated secondary antibody (either biotin-donkey anti-goat IgG Fab2 diluted
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