Rabbit Red Blood Cell Suspensi

Subculture of Suspension Cell Lines

of Suspension Cell Lines Click here for a schematic diagram of "Subculture of Suspension Cell Lines" Aim In general terms cultures derived from blood (e.g. lymphocytes) grow in suspension. Cells may grow as single cells or in clumps (e.g. EBV transformed

Human Peripheral Blood Mononuclear Cell Preparation

in 1 to 2 ml of PBS, and transfer the latter to a fresh tube (to avoid contaminating the solution with the platelets that are adhering to the sides of the tube).7. Bring the cell suspension to a final volume of 50 ml and gently overlay 12.5 ml onto the Ficoll

Cell Cultures

of antibodies, which might negatively effect cell growth.Our first procedure will involve the simple transfer of an established culture from a suspension culture. An aliquot will be removed from a commercially available cell line and transferred to prepared