Thioredoxin-like protein HCF16

Identification of Thioredoxin Target Disulfides Using Isotope-Coded Affinity Tags

quantification of Trx target disulfides in complex protein extracts is described. The procedure utilizes the isotope-coded affinity tag (ICAT) reagents containing a thiol reactive iodoacetamide group and a biotin affinity tag to target peptides containing reduced

Protein A Mimetic (PAM) Affinity Chromatography: Immunoglobulins Purification

Antibodies of the G class can be conveniently purified, even at large scale, by affinity chromatography using immobilized protein A or G. Because specific and cost-effective ligands are not available, scaling up purification of immunoglobin

Preparation of N-GST Fusion Protein for Affinity Immobilization of RNA

bound to glutathione-Sepharose resin allows affinity purification of RNA with very high purity and yield. This Chapter outlines the experimental procedure employed to prepare the λN-GST fusion protein used for RNA immobilization in successful affinity