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- 2025年07月13日
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文献和实验Steady State ATPase Assays Coupled Enzyme System
Motor protein (>95% purity; 15-20 µM) Cuvettes (200 µL volume, 10 mm path length) 0.5 M Tris-OAc, pH 7.5 10 mM MgCl2 10 mM DTT
ELISA Procedure for Measuring Serum Antibody Titer
assays are the most appropriate for measuring the titer of the antisera you have generated. ELISA Procedure for Measuring Serum Antibody Titer In this type of ELISA (Enzyme-Linked Immunosorbant Assay), the antigen (peptide or protein) is bound
ELISA (Enzyme-Linked ImmunoadSorbent Assay)
for at least 1hr at 37°C. 5) Remove BSA by flicking plate. 6) Wash 3x with 1x PBS containing 0.01% tween 7) Add anti-serum (diluted as appropiate, 1/100 etc. with 1x PBS) and incubate for 2hrs @ 37°C. N.B.: Use positive and negative controls. 8) Wash 4x
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