- ￥9480
- Cedarlane
- CLAS15-3002
- 2025年07月13日
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 库存：
999
- 规格：
EA
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验Quantitative immunoprecipitation of GFP-fusion proteins using the GFP-Trap
proteins / chromatin proteins: DNaseI (f.c) 1 µg/ µl 2.5 mM MgCl2 RIPA Buffer 10 mM Tris/Cl pH 7.5 150 mM NaCl 5 mM EDTA 0.1% SDS 1% Triton X-100 1% Deoxycholate 1 mM PMSF
Measurement of GFP Expression and DNA Content in Permeabilized Cells
supernatant by aspiration or rapid decanting and add 1 ml of a solution containing 40µg/ml of PI and 100 µg/ml of ribonuclease A. Incubate cell suspension at 37o C in the dark for 30 min. If needed, filter samples through a nylon mesh to remove clumps
in yeast We tested mTn-3xHA/GFP by mutagenesis of the BDF1 gene, which encodes a chromatin-associated protein. We grew individual bdf1::mTn-3xHA/GFP transformants to a density of 107 cells/ml in SC-ura. The last four hours of growth were at room
技术资料暂无技术资料 索取技术资料
