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文献和实验Single-Molecule Tracking of Carbohydrate-Binding Modules on Cellulose Using Fluorescence Microscopy
Fluorescence Microscopy (TIRF-M) to visualize single molecules of carbohydrate-binding module (CBM) labeled with green fluorescent protein (GFP). The content describes step-by-step sample preparation and data acquisition, processing, and analysis. These methods
was measured in front-face configuration. The measured fluorescence signal was a result of a specific interaction. To verify this, we performed the immunoassay using the same labeled antibodies, but with a nonspecific antigen, rabbit IgG
Methods for use with the mTn-3xHA/GFP-mutagenized library
were at room temperature, to allow formation of the GFP chromophore. Then we examined cells directly using a Leitz microscopy with a system 13 filter (this may not be optimal). In 4 of 38 transformants, we saw green fluorescence of the nucleus. Fixation
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