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- 2025年07月01日
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- 详细信息
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50 µl
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文献和实验serum (FBS;HyClone, Ogden, UT) and L -glutamine (Gibco/BRL). 2. Phosphate-buffered saline (PBS) containing 0.5 M ethylenediaminetetraacetic acid (EDTA). 3. Solution of trypsin (0.25%) containing 1 mM EDTA (Gibco/BRL). 4. Western lysis buffer
on silver film. 2. Alexa488 Model Immunoassay Mouse and rabbit IgGs, buffer components and salts (such as bovine serum albimun, sucrose) were from Sigma-Aldrich. Bocking solution was 1% bovine serum albumin, 1% sucrose, 0.05% NaN3 , 0.05% Tween
IF protocol for perfusion fixed tissue
saturated picric acid in 0.1 M. phosphate buffer pH 7.4. Brains incubated in 20w/v sucrose in 0.1 M PB (24 hours at 4°C) followed by freezing in isopentane (-40°C). 30 micron coronal sections cut on freezing microtome/cryostat for free floating IF.
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