T7, DyLight® 550 conjugated,(H
ost: Rabbit),(Purity: Affinity purified),(Size: 100 µg (1mg/ml))- ￥10120
- Cedarlane
- CLAS15-2984
- 2025年07月16日
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文献和实验Magnetic DNA Affinity Purification of a Cellular Transcription Factor
genes (2 –8 ) and is tethered to EBNA2 responsive promoter elements by interaction with a cellular DNA binding protein (9 ,10 ). We purified this transcription factor by classical chromatographic methods, however, instead of the usual final DNA
RUVKUN Antibody Staining Protocol revised 10/29/90
: 11. Can preincubate in Buffer A (1% BSA) at RT for a few hours. 12. Spin down worms gently and incubate with 1 in Buffer A (30 ul affinity purified anti-lin-14 antibody -preadsorbed with 292 vector protein- straight or diluted 1:1 or 1:2) for 24
Mapping Protein Distributions on Polytene Chromosomes by Immunostaining
Reagents Ammonium sulfate (50% [w/v]) (optional, see Step 23) Antibodies, primary Affinity-purified primary antibodies are diluted in PBS containing 1% BSA. The dilutions must be adjusted for each individual primary antibody. Typical
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