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文献和实验on silver film. 2. Alexa488 Model Immunoassay Mouse and rabbit IgGs, buffer components and salts (such as bovine serum albimun, sucrose) were from Sigma-Aldrich. Bocking solution was 1% bovine serum albumin, 1% sucrose, 0.05% NaN3 , 0.05% Tween
secondary antibody review -- data from 99 publications
ref host class subclass conjugation experiment dilution detail supplier 1 goat Alexa Fluor 488 immunocytochemistry 1:250 localization of tagged EVA-1 in 293T cell Molecular Probes 1 goat Alexa Fluor 546 immunocytochemistry 1:250
, and 2) fractionation over BlueSepharose, which binds Wnts with high selectivity. The purificationconsists of four chromatography steps: Blue Sepharose, immobilized metal affinity chromatography (IMAC), gel filtration,and heparin cation exchange
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