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文献和实验Platelet-associated Ig (PAIg) Elution
OUTLINE Platelet-associated Ig (PAIg) could be eluted from the platelets. As a result of this procedure platelets get destroyed and the eluted Ig is formed of platelet surface-bound Ig and , mainly, of internal
Antigen Design Sera Purification Tech Sheet
subcutaneously at multiple sites. A pre-immune bleed should be drawn from each host animal to produce a baseline to which the production bleeds can be compared. The drawn sera will contain a number of different types (IgG, IgM, IgA) and subclasses (Ig1, Ig2a, Ig
this method will dilute the antibody by a factor of 2. Using a PD-10 column: a)Wash PD-10 column with 30mL of PBS. b) Add the 1mL FITC-Ig or Biotin Ig solution after the top of column is almost dry. Close stopcock to stop flow after the Antibody
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