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文献和实验Mapping Protein Distributions on Polytene Chromosomes by Immunostaining
serum, obtained from the same species as the secondary antibody. Depending on the method employed, use anti-rabbit IgG (Fc) horseradish peroxidase (HRP) conjugate (1:100 dilution), a biotin-conjugated secondary antibody, or a fluorescence-conjugated
16-27) describes how to immunopurify RNA-protein complexes from worm extract in two steps. First, the extract is incubated with the antibodies to allow binding of the antibody to its target protein. Second, the antibody with the associated protein
RNA-chromatin immunoprecipitations (RNA-ChIP) in mammalian cells
and proteins in the host mammalian cell (Reference 3). In RNA-ChIP, RNA-protein interactions are fixed by reversible chemical cross-linking with formaldehyde followed by immunoprecipitation with antibodies against the candidate protein(s). RNAs
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