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文献和实验Strategies to Optimize Protein Expression in E. coli
with affinity tag: Expression from Saccharomyces cerevisiae and its utilities in fusion protein technology. Biotechnol. Bioeng. 75:718‐724. Chong, S.R., Montello, G.E., Zhang, A.H
Antigen Design Sera Purification Tech Sheet
, it is important to avoid self-recognition of the immunogen by the host animal. As an example, when raising antibodies against a human protein, it is more suitable to use a rabbit or mouse host than a monkey. For highly conserved mammalian proteins, raising
-RNA complex is affinity purified using Protein A-agarose. Perform Steps 16-27 at 4°C unless otherwise stated. 16. For each IP experiment (consisting of one specific and one nonspecific antibody sample), place 60 µL of bed
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