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文献和实验Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture
serum. 7. Scrape the cells from the plate. Transfer them to 50-mL tubes. Optionally, scrape a second time with PBS containing protease inhibitor cocktail. 8. Collect the cells by centrifuging at 1300 rpm for 8 min at 4°C
ELISA Procedure for Measuring Serum Antibody Titer
reader Pipette tips Solutions to Prepare for the ELISA ProcedureSodium carbonate (Na2CO3) buffer, 50 mM, pH 9.6 with 0.02% NaN3 PBS-T (PBS containing 0.05% Tween-20) 3% BSA in PBS-T Sodium acetate (NaOAc), 0.1 M ELISA Procedure Run duplicates
Protein A Mimetic (PAM) Affinity Chromatography: Immunoglobulins Purification
(3 ) and represents the second most abundant Ig in serum (4 ), can be purified by classical chromatographic approaches with an acceptable degree of purity. But several steps, such as ammonium sulfate precipitation, ion-exchange chromatography, and gel filtration
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