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文献和实验Protein A Mimetic (PAM) Affinity Chromatography: Immunoglobulins Purification
Antibodies of the G class can be conveniently purified, even at large scale, by affinity chromatography using immobilized protein A or G. Because specific and cost-effective ligands are not available, scaling up purification of immunoglobin
Magnetic DNA Affinity Purification of a Cellular Transcription Factor
affinity column step, we applied a magnetic DNA affinity purification protocol. This increased the purity of the transcription factor from about 0.01% to almost homogeneity and allowed subsequent identification of the protein as RBP-Jκ (11 ).
Immobilized Metal Ion Affinity Chromatography of Histidine-Tagged Fusion Proteins
and its cognate antigen, IMAC can sometimes display non-ideal product purity. The protocols described in this chapter provide strategies for the improvement in the purity of IMAC-purified proteins. Similarly, non-specific binding May reduce product yields
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