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文献和实验Protein A Mimetic (PAM) Affinity Chromatography: Immunoglobulins Purification
into the purified preparation. Second, jacalin binds to the carbohydrate moiety of IgA, and D-galactose is required to elute IgA from affinity columns, which is costly and impractical for large-scale operations.
Cloning of PLD2 from Baculovirus for Studies in Inflammatory Responses
, and anti-apoptosis. We describe in the present study molecular, cellular, and physiological methods to understand the mechanism of how the PLD2 isozyme regulates the process of inflammation. We describe here (1) a method that details phospholipase D2 (PLD
Purification of DNA‐Binding Proteins Using Biotin/Streptavidin Affinity Systems
of DNA‐binding proteins using the biotin/streptavidin affinity technique. The protocol involves the following steps: (1) a biotinylated, labeled DNA fragment is prepared containing a binding site for the protein to be purified; (2) the biotin‐cellulose
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