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文献和实验Studies of the Ubiquitin Proteasome System
Assays of E1 and E2 Activity Basic Protocol 1: Thiolester Formation Between Rabbit E1 and Ubiquitin Basic Protocol 2: Thiolester Formation Between E2 and Ubiquitin Binding of Ubiquitin‐Proteasome Proteins Basic Protocol 3: Binding of E2s to E3s Alternate
Measurement of Oxygen Radicals and Lipid Peroxidation in Neural Tissues
8‐Isoprostane EIA kit (8‐iso‐PGF 2α ; Cayman Chemical) containing: Precoated (mouse anti‐rabbit IgG) EIA 96‐well strip plates
Strategies to Optimize Protein Expression in E. coli
in Escherichia coli (E. coli ) is simple, fast, inexpensive, and robust, with the expressed protein comprising up to 50 percent of the total cellular protein. However, it also has disadvantages. For example, the rapidity of bacterial protein expression
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