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- Cedarlane
- CLAS04-042S
- 2025年07月13日
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文献和实验STANDARD PLANT MOLECULAR BIOLOGY PROTOCOLS
if aliquoted. Do not mix before use. Positive control uses 10ng supercoiled plasmid END LABELLED PROBE PREPARATION 1) Digest plasmid w/ 5''overhang enzyme 2) P/CHCl3 ext 3) EtOH ppt 4) Redissolve to 0.5mg/ml in H2O 5) Start
Standard Operating Procedures for T1-Phage Testing Assay
lawn, the lawn there will be lysed on incubation overnight. II. Materials and Equipments: DH10B (original glycerol stock) or DH10B cultured stock for phage test. DH10B-T1 phage infected cells (positive control).
to the calibrator. As an example, in a study of drug effects on expression, the untreated control would be an appropriate calibrator. Critical Guidelines The guidelines below are critical for proper use of the standard curve method for relative quantitation
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