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文献和实验Cloning and Culturing of Fetal Mouse Natural Killer Cells
and functional capabilities of “individual” T cells. More recently, the development of procedures for the cloning of human NK cells was instrumental in the discovery of killer cell immunoglobulin-like inhibitory (KIR) receptors (2 , 3 ; and Chapter 1 ).
Generation of Antibodies to Cell Surface Markers on Mature Natural Killer Cells
non-Ly49 receptors for class I antigens. Molecules similar to human killer cell immunoglobulin-like receptors (KIRs) have not been cloned in mice. Other receptors receive activating or positive signals and therefore may have important NK cell functions
immunoglobulin-coated magnetic beads. Remaining contaminating cells can be depleted using a combination of specific biotinylated antibodies and anti-biotin-coated magnetic beads, in the case of natural killer (NK) cells, or by FACS gating, during sorting
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