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文献和实验ELISA Procedure for Measuring Serum Antibody Titer
Phosphatase Reagents for Alkaline Phosphatase DetectionGoat anti-species IgG whole molecule/alkaline phosphatase conjugate that is appropriate for the source of your antiserum. For example, if the antibodies were raised in a rabbit, use goat anti-rabbit IgG
Clinical Chemistry and Other Laboratory Tests on Mouse Plasma or Serum
/) 96‐well plate (8 well removable strips) coated with polyclonal rabbit anti‐PINP antibody (from Competitive ELISA kit; IDS Ltd, http://www.idsplc.com/) Precision
Polyclonal and Monoclonal Antibodies
, boost again a month later). 3) 7 - 10 days later bleed the mouse and test serum using the assay which will be used for screening. If titre is not high enough, boost again two weeks after previous boost. Serum Preparation:
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