Anti-Mouse NK Cells (NK1.1),

Hybrid Resistance by Mouse NK Cells In Vitro

, leads to generation of lytic NK1.1+ Ly49- cells whose function is similar to that of fetal NK cells, i.e., they can lyse YAC-1 tumor cells and class I deficient cells, but cannot lyse normal lymphoblasts and lack Ly49 receptors (5 ). These two types

Subset Analysis of Human and Mouse Mature NK Cells

Identification of natural killer (NK) cell subsets has gained attention with the recent discovery that mature mouse NK cells comprise two distinct stages. Delineation of the stages is performed using the markers CD27 and CD11b on gated NK

Assessing Licensing of NK Cells

, splenocytes are stimulated with plate-bound anti-NK1.1 monoclonal antibody in the presence of brefeldin A and then assessed for NK cell activation on a single-cell basis using intracellular cytokine interferon-γ staining and flow cytometry.