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文献和实验Assay of Anti-Endotoxin Antibodies
the endotoxin of Gram-negative bacteria. In mammals, including humans, LPS exhibits a variety of biological effects that may be beneficial if administered in low amounts but harmful when present in higher concentrations as in the case of Gram-negative infection
Transgenic Mouse and Transgenic Rat Outline
into fertilized mouse or rat eggs, the Transgenic Core requires that you provide evidence that you have a PCR or Southern blot assay that detects your transgene when it is spiked into tail DNA at a one copy concentration. A second assay that will detect
Purification of Endotoxin Free mAbs
is applicable to most rat and mouse IgG antibodies (not IgM), although individual monoclonal antibodies may require modification of either the pH or ionic strength for optimal purification. Cation exchange using Fast Flow S-Sepharose (Sigma S 1264) at pH
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