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文献和实验PRIMARY MOUSE KERATINOCYTE CULTURES Isolation of epidermal keratinocytes from neonatal mice is based on the protocol of Dlugosz et al., Methods Enzymol . 254:3-20 (1995). The epidermis from a newborn mouse should yield
Hybrid Resistance by Mouse NK Cells In Vitro
, which suggest that inhibitory non-Ly49 NK receptors are expressed on such NK cells and that these receptors can receive negative signals from class I molecules (3 ,4 ). The stimulation of bone marrow NK precursor cells with various cytokines, including IL-15
Culturing Mouse Embryonic Fibroblasts
Dissecting tools: Scissors, forceps, scalpel 95% ethanol in a squeeze bottle and in a beaker Mouse breeders Note: This protocol describes the isolation of primary MEFs and passage according to the 3T3 protocol. Passaging primary cells
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