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文献和实验4.1 ), using 15% acrylamide (15%T:5%C) in 2.5 M urea and 0.9 M acetic acid. Set up the gel in the electrophoresis unit and run the gel at 2 mA/gel for 2 hours with no sample, using 0.9 M acetic acid for the running buffer. Dissolve
Single tube confirmation PCR protocol
for each of the 3 isolates and the wild-type control. - Incubate the Zymolyase solutions at 37 ° C for 30 minutes followed by 10 minutes at 95 ° C. *It is not necessary to remove the Zymolyase solution by pelleting the cells before the PCR. *The Zymolyase
production (ELISA). Freeze strong positive wells (as given below), and expand the cell line in vitro using a large tissue culture flask or in vivo by initiation of ascites fluid in mice (BALB/c).
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