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文献和实验Protein Therapeutics: Mouse, Humanized, and Human Antibodies
component of the vertebrate immune system. Each plasma cell secretes a single clone of antibodies that bind a unique epitope of an antigen. Cooperations exist between antibodies and other immune effectors such as macrophages and complement to facilitate
Th17 Polarization of Mouse CD4 Cells
实验试剂 Sterile PBS Cell culture medium Sterile plastic petridishes RBC Lysis Buffer [Details: Biolengend, cat.#420301] Anti-mouse CD3ε, clone145-2C11[Details: LEAF™format, cat.#100314]
Human Antibodies by Guided Selection
. This chapter will describe the sequential guided selection procedure using a Fab format as an example. The first step is to clone the variable regions of a mouse antibody to a phagemid vector containing human constant domains, C? and CH1, which results
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