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文献和实验Protein Therapeutics: Mouse, Humanized, and Human Antibodies
component of the vertebrate immune system. Each plasma cell secretes a single clone of antibodies that bind a unique epitope of an antigen. Cooperations exist between antibodies and other immune effectors such as macrophages and complement to facilitate
Purification and Characterization of Human and Mouse Complement C3
. This chapter describes the purification of C3 from human and mouse plasma using protein precipitation, followed by classical ion exchange chromatography and gel filtration. The biochemical and functional characteristics of the purified C3 are typically assessed
-purified goat anti-mouse IgG (Jackson Labs; 1 mg/ml stock used at 1:300 dilution) Texas Red-conjugated affinity-purified donkey anti-rabbit (Jackson Labs;1 mg/ml stock used at 1:200 dilution)
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