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文献和实验Th17 Polarization of Mouse CD4 Cells
实验试剂 Sterile PBS Cell culture medium Sterile plastic petridishes RBC Lysis Buffer [Details: Biolengend, cat.#420301] Anti-mouse CD3ε, clone145-2C11[Details: LEAF™format, cat.#100314]
strains. 1. Use C57BL/6 F1 mice to set up new breeding cages (see mouse breeding suggestions above). Most C57BL/6 F1 hybrids have good reproductive performance. We prefer (C57BL/6J X DBA/2J)F1 animals. Outbred CD-1 and ICR mice can be used
Transgenic Mouse and Transgenic Rat Outline
-galactosidase or human placental alkaline phosphatase. You may use a non-mouse protein such as a human protein or an epitope tagged (i.e. HA, FLAG or myc) protein that can be identified with an antibody that doesn't bind to mouse proteins. For further discussion
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