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文献和实验Measurement of Nitric Oxide in Single Cells and Tissue Using a Porphyrinic Microsensor
(see recipe ) Biological matrix: cells (grown on 25‐mm tissue culture dish) or tissue of interest Chemical agonist
The OP9-DL1 System: Generation of T-Lymphocytes from Embryonic or Hematopoietic Stem Cells In Vitro
reagent; BD Biosciences 555899) (for Protocol 4) Buffer for cell staining and cell sorting (for Protocol 4; see Step 72) Complement, reconstituted from rabbit (Cedarlane CL3331) (for Protocol 3) ESC medium (for Protocols 1, 2) Freezing
Routine Splitting and freezing of cells
tissue culture grade eg. DMSO Sigma D-2650, aliquoted and frozen at -70�). Filter DMSO / media prior to resuspending cells in it. 4. Add cells to Nunc cryotubes (1ml ea. Cat. 3-63401) and label with initials, experiment no. and cell name in pencil
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