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- CL20040F
- 2025年07月11日
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文献和实验Dual- and Triple-Color Fluorospot
labeled with green fluorophore emitting light at the same wavelength as FITC, streptavidin (SA) labeled with Cy3, and if three cytokines are analyzed, a mouse anti-tag mAb labeled with Cy5 (Mabtech). 5. Mouse mAb anti-human CD28 at 0.1 mg/ml
Reagents: NGS-Normal Goat Serum PBST-0.5% Tween-20/CMF-PBS CMF-PBS 1°Ab-mouse MF20 (anti-myosin monoclonal) 1°Ab-rabbit myoD (anti-myoD polyclonal) 2°Ab-goat anti-mouse IgG-FITC 2°Ab-goat anti-rabbit IgG-FITC
Immunohistochemisty-Fluorescence Protocol
and Secondary Reagents Biotinylated donkey anti-goat IgG (Jackson Immuno Research Lab Catalog # 705-066-147) Biotinylated goat anti-mouse IgG1 (Caltag Lab Catalog # M32115) FITC-labeled goat anti-mouse IgG1 (Caltag Lab Catalog # M32001)
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