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文献和实验Acid Adjusted Swine Serum 1. The before or morning of: adjust the pH of the swine serum to between 4.3 and 4.5 with 1N HCl. 2. Place serum for at least 1 hour at 4°C (overnight is best). 3. Centrifuge serum at 6,000 rpm (Beckman centrifuge, 250
Resuscitation of Frozen Cell Lines
Aim Many cultures obtained from a culture collection, such as ECACC, will arrive frozen and in order to use them the cells must be thawed and put into culture. It is vital to thaw cells correctly in order to maintain the viability
Method: Lymphoblastoid Cell Lines from Frozen Whole Blood
Method: Lymphoblastoid Cell Lines from Frozen Whole Blood May 31, 1990 Rosalie Veile Purpose: Blood Samples can be stored frozen as a backup in case an LCL is needed at a later date. Time
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