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文献和实验Transgenic Mouse and Transgenic Rat Outline
an endogenous mouse gene, such as beta-globin, or an endogenous rat gene, such as prolactin, is required in order to demonstrate that the DNA preparations are free of PCR inhibitors. Animals are tested with both assays so that no transgenic founder is mistakenly
Designing TCR for Cancer Immunotherapy
the circumvention of tolerance to tumor-associated (self) antigens (TAA). This chapter reports on the identification of the α and β chains of the heterodimeric TCR derived from a mouse T-cell clone. The related DNA fragments are inserted into a retroviral vector
Cloning and Expression of Single-Chain Fragments (scFv) from Mouse and Rat Hybridomas
, including rat hybridomas (6 –8 ). However, some of the restriction sites (in particular Pst I and Barn HI) introduced for subsequent cloning were found to be frequently present as internal sites in the DNA coding for mouse-antibody-variable domains
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