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文献和实验Assessing Licensing of NK Cells
Natural killer (NK) cells express receptors to detect and kill target cells based on expression of target cell surface molecules. Through a process termed NK cell licensing, only NK cells that express inhibitory receptors (e.g., Ly49
Introduction of shRNAs into Primary NK Cells with Lentivirus
differentiation, acquisition of self-tolerance, and induction of anti-tumor responses. We used VSV-G-pseudotyped, EGFP-expressing lentiviral vectors to develop an efficient gene transfer system to modify gene expression in primary murine NK cells
Fusion of Mouse, Rat, or Hamster Cell
. Collect the cells into a 50 ml tube. Wash the Petri dish with an additional 10 ml sterile PBS and add to the tube. Allow the cells to settle for 1 minutes. Carefully remove the cell
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