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文献和实验The OP9-DL1 System: Generation of T-Lymphocytes from Embryonic or Hematopoietic Stem Cells In Vitro
complement and either 5 mL of culture supernatant containing anti-CD24 mAb (J11d clone) or 5 ml of OP9 medium containing 1 ng of purified anti-CD24 mAb per 106 cells. CD24 is expressed in all Lineage + cells in E13-E15 fetal liver cells
Phycoerythrin conjugation protocol
, about 2.5 mg/ml PE R-Phycoerythrin, purified (Molecular Probes P-801 in 60% ammonium sulfate) PBS Phosphate buffered saline without Ca2+ /Mg2+ MeOH Methanol
Intracellular Cytokine Staining Protocol
anti-cytokine antibodies with excess amounts of unlabeled antibody. Alternatively, recombinant cytokines can be used for blocking.eBioscience antibodies are available as Functional Grade (FG) Purified (sterile, endotoxin-tested, no azide), Purified
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