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文献和实验Transgenic Mouse and Transgenic Rat Outline
into fertilized mouse or rat eggs, the Transgenic Core requires that you provide evidence that you have a PCR or Southern blot assay that detects your transgene when it is spiked into tail DNA at a one copy concentration. A second assay that will detect
Fusion of Mouse, Rat, or Hamster Cell
Preparation of Myeloma Cells. Collect myeloma cells from 2-4 T-150 flasks in log phase of growth. Wash twice with sterile PBS. Resuspend the cells in 10 ml
The OP9-DL1 System: Generation of T-Lymphocytes from Embryonic or Hematopoietic Stem Cells In Vitro
N 3M5, Canada 1 Corresponding author (jczp@sri.utoronto.ca ) INTRODUCTION Differentiation of mouse embryonic stem cells (ESCs) or hematopoietic stem cells (HSCs) from fetal liver or bone marrow into T-lymphocytes can be achieved in vitro
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