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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20°-20°
- 保质期:
1-3年
- 库存:
999
- 供应商:
亦高生物
- 规格:
AB144P-500ul

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文献和实验1.5M Tris(PH 8.8) 100ml 18.171g (at4°C) 10% SDS 100 ml 10g (at room temperature) 10% ammonium persulfate 1ml 0.1g(replace every week store at at4°C) TEMED 8 room temprature Antibodies Primary antibody: 5A11(anti-mouse IgG) 500x-1000x Secondary
The production of monoclonal antibody by hybridoma
The production of monoclonal antibody by hybridoma fusion: Immortalization of sensitized B lymphocytes from immune mice. Overview
Large-scale immunofluorescence
) or orbital platform shaker (240 r.p.m.). Centrifuge microtiter dishes for 5 minutes at 500 X g to pellet cells. Fix cells by adding 1/10 volume of 37% formaldehyde and incubating at room temperature for 15 minutes to 1 hour 2 without shaking
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