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DNA Ligase III rabbit pAb

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  • ¥900
  • AAB
  • 上海
  • A13648
  • 2025年07月05日
  • WB,ELISA
  • Rabbit
  • Human,Mouse,Rat
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 免疫原

      Synthesized peptide derived from DNA Ligase III . at AA range: 110-190

    • 形态

      Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

    • 保存条件

      -20°C/1 year

    • 克隆性

      Polyclonal

    • 适应物种

      Human,Mouse,Rat

    • 保质期

      1年

    • 宿主

      Rabbit

    • 应用范围

      WB,ELISA

    • 浓度

      1 mg/ml

    • 靶点

      LIG3

    • 抗体英文名

      DNA Ligase III rabbit pAb

    • 抗体名

      DNA ligase 3

    • 规格

      50ul

    DNA ligase 3(LIG3) Homo sapiens This gene is a member of the DNA ligase family. Each member of this family encodes a protein that catalyzes the joining of DNA ends but they each have a distinct role in DNA metabolism. The protein encoded by this gene is involved in excision repair and is located in both the mitochondria and nucleus, with translation initiation from the upstream start codon allowing for transport to the mitochondria and translation initiation from a downstream start codon allowing for transport to the nucleus. Additionally, alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008],

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    图标文献和实验
    相关实验
    • OGMP - Organelle DNA Library Construction

      2 units T7 DNA polymerase 1.5 ul Klenow buffer (10x) --------------------------- in 15 ul final volume. Incubate for 30 min at 12'C. Heat for 5 min to 65'C, let cool down. III. SIZING 1. Separate on LM agarose gel

    • 细胞器DNA文库构建

      I. NEBULIZATION of DNA 1. 0.5 - 5 ug DNA in TE (10mM/1mM), 25% glycerol, final volume 500 ul 2. nebulize for 90-100 sec at 5-10 psi (for a medium size of about 1.5kbp) 3. Precipitate with EtOH/Ammonium Acetate

    • Organelle DNA Library Construction

      down for 30 min). Dissolve pellet in 10 ul TE. IV. PHOSPHORYLATION 10 ul sized DNA 2 ul rATP (stock : 10 mM) 2 ul ligase-buffer 10x (similar to kinase buffer) 5 units kinase --------------- in 20 ul

    图标技术资料

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