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His-Tag mouse mAb(4E6)

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  • ¥900
  • AAB
  • 上海
  • A42021
  • 2025年07月11日
  • WB,IP,IF
  • Mouse
  • Species independent
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 免疫原

      Synthetic peptide:HHHHHH conjugated to KLH.

    • 形态

      PBS, pH 7.4, containing 0.5%BSA, 0.02% sodium azide as Preservative and 50% Glycerol.

    • 保存条件

      -20°C/1 year

    • 克隆性

      Monoclonal

    • 适应物种

      Species independent

    • 保质期

      一年

    • 宿主

      Mouse

    • 应用范围

      WB,IP,IF

    • 浓度

      1 mg/ml

    • 抗体英文名

      His-Tag mouse mAb(4E6)

    • 抗体名

      His Tag

    • 规格

      100ul

    A polyhistidine-tag is an amino acid motif in proteins that consists of at least five histidine (His) residues, often at the N- or C-terminus of the protein. It is also known as hexa histidine-tag, 6xHis-tag, and by the trademarked name His-tag. Polyhistidine-tags are often used for affinity purification of polyhistidine-tagged recombinant proteins expressed in Escherichia coli and other prokaryotic expression systems.

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    图标文献和实验
    相关实验
    • Purification of mAb (IgG)

      (adjust pH to 7.8 with Binding buffer; red color) to the Protein A column.Mouse antibodies of the IgG1 subclass do not have a high affinity for protein A. Purification on protein A beads using standard conditions will yield approximately 1/10

    • Purification of mAb (IgG)

        Purification of mAb (IgG) by Chang-Duk Jun, 03/14/2000 Purpose Materials Antibody 7E3 , 2L sup grown in flasks, frozen and thawed overnight. BioRad Affi-Gel Protein A MAPS II Buffers

    • T-Cell Activation Using mAb to CD3

      One of the most common ways to assess T cell activation is to measure T cell proliferation upon in vitro stimulation of T cells via antigen or agonistic antibodies to TCR. This protocol is written as a starting point for examining in vitro proliferation of mouse

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