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文献和实验Fastfilter Plasmid Maxi Kit Spin Protocol
. All of centrifugation steps must be carried out at room temperature. Purify Plasmid DNA with HiBindTM DNA Maxi Column: 10. Transfer 16 ml of the cleared lysate to the HiBind® DNA maxi column assembled in the 50 ml collection tube. The Maxi column
1. Weigh 500 mg of glass beads in a 2 ml centrifuge tube, add 0.2-0.5 g soil sample. Add 0.8 ml Buffer SLX Mlus. Vortex at maximum speed for 3-5 minute to lyse samples. For the best result, A Mixer Mill, such as Fastprep-24, Mixer Mill MM 300
Using Soil DNA Kit (D5625) to Clean Up DNA containing inhibitors
3. Centrifuge at full speed (>13,000 x g) for 2 minutes. 4. Transfer cleared supernatant to a new 1.5 ml tube. Note: If the supernatant still shows dark color from soil at this point, perform the HTR extraction again by repeating step 1-4.
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