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- 2025年07月09日
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文献和实验Fastfilter Plasmid Midi Kit Spin Protocol
with agitation for 12-16 h. For best results use overnight culture as the inoculum. It is strongly recommended that an endA negative strain of E.coli be used for routine plasmid isolation. Examples of such strains include DH5α® and JM109® . Optimal
RNA Isolation From Animal tissue or cell culture
empty, centrifuge the column for 2 min at full speed ($13,000 x g) to completely dry the HiBind® matrix. 11. Elution of RNA. Transfer the column to a clean 1.5 mL microfuge tube (not supplied with kit) and elute the RNA with 15-20 ul of DEPC-treated water
DNA/RNA/Protein Purification from Cultured Cells Using SQ DNA/RNA/Protein Cell Kit (1-2 x 106 cells)
the centrifuge at 4°C for RNA isolation. 5. Frozen cells should be thawed quickly using a 37°C water bath with gently agitation and place on ice until use. 6. Warm up the Cell Lysis Buffer at 37°C water bath Procedure 1. Harvest and lyse
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