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文献和实验empty, centrifuge the Midi column for 10 min at >4000 x g to completely dry the HiBind™ matrix. 9. Elution of RNA. Transfer the column to a new RNase-free 15 ml centrifuge tube (not supplied with kit) and elute the RNA with 0.3-0.5 ml of DEPC water (supplied
RNASafer® Protocol for Total RNA Isolation
buffer. Homogenize the tissue with a rotor-stator homogenizer. For RNA isolation using Midi and Maxiprep kit, follow the standard protocol in the user manual. 4) Follow standard protocol for E.Z.N.A.® Total RNA Kits in user manuals.
Do Your Experiments Require Total RNA or mRNA? 看看就会有收获 Selecting a Purification Strategy . . . . . . . . . . . . . . . .. . . . . . . . . 198 Do Your Experiments Require Total RNA or mRNA? . . . . . 198 Is It Possible to Predict the Total RNA
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