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- 2025年07月15日
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文献和实验Streptomyces:Protocols/Mini-Maxi Prep
Small Scale Plasmid Isolation (Mini / Maxi Prep) Description A mini prep / maxi prep is used to isolate plasmid or cosmid DNA from bacteria, normally E.coli. An overnight culture of cells is lysed and the majority of the cells
from the handbook and annotated based on experience with the kit. Protocol: QIAprep Spin Miniprep Kit Using a Microcentrifuge This protocol is designed for purification of up to 20 μg of high-copy plasmid DNA from 1�5 ml overnight cultures of E. coli in LB
E.Z.N.A. Endo-Free Plasmid Mini Kit II Spin Protocol
until a flocculent white precipitate forms. Centrifuge at maxi speed (13,000 x g) for 10 minutes at room temperature. NOTE: The Buffers must be mixed throughly. If the mixture appears still viscous, brownish and conglobated, more mixing is required
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