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文献和实验On-chip Isotachophoresis for Separation of Ions and Purification of Nucleic Acids
the channel. 4. Extraction and purification of nucleic acids from cultured E. coli The ability to selectively focus ionic species makes ITP an ideal technique for biological sample preparation. We purify nucleic acids from untreated cell lysate
DNA/RNA/Protein Purification from Cultured Cells Using SQ DNA/RNA/Protein Cell Kit (1-2 x 106 cells)
. For Total Nucleic acid isolation, proceed step 8-12. For RNA and DNA isolation, proceed step 13-25. Total Nucleic acid Isolation: 8. Add equal volume (400 μL) of isopropanol. Mix throughly by vortexing for 30 seconds. Incubate the tube at room
E.Z.N.A.® Viral RNA Spin Protocol
, Carrier RNA and 2-mercaptoethanol can be premixed together. The premixed lysis buffer can stored at 2-8°C for 1 week. Increase the amount of Lysis Buffer proportionally if the sample volume is larger than 140 ul. 2. Pipet 140 ul plasma, cell free
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