Detergent-Free Total Protein I

Isolation of Total RNA from Difficult Tissues

techniques to help overcome problems associated with isolating total RNA from difficult tissues. The tips provided are based on RNA isolation by a guanidinium thiocyanate/acid phenol:chloroform extraction method (e.g. Ambion"s ToTALLY RNA™ Kit). Note

Isolation of Total RNA from Animal Cells use RNeasy Mini Kit

3)   Transfer cells to an RNase-free centrifuge tube and pellet by centrifugation at    300 x g for 5 min. (In our lab, use 1800rpm in the eppendorf centrifuge 5415D). Cell pellets can be stored at –70°C for later use or used directly in the procedure

The Extraction of Total RNA by the Detergent and Phenol Method

that minimizes degradation of the RNA by hydrolysis or ribonuclease activity. The method described here relies on cell homogenization in an aqueous medium containing a strong detergent (sodium tri-isopropylnaphthalene sulfonate) and a chelating agent (sodium