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- 2025年07月12日
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文献和实验uses qRT-PCR as the method of choice, and Applied Biosystems TaqMan Gene Expression Assays provide validated, easy-to-use primer-probe sets for this purpose (Figures 3 and 4). The Silencer GAPDH siRNA Control--and the TaqMan Gene Expression Assay
Real-Time PCR for Specific Detection of Three Phytoplasmas from the Apple Proliferation Group
of assays comprises the same set of primers and specific probes for species-specific amplification, and an additional set of primers and probe for 18S rRNA as an endogenous quality control of DNA extraction. The assays described can be used in routine
and exogenous controls are examples of active references. Active reference means the signal is generated as the result of PCR amplification. The active reference has its own set of primers and probe. Endogenous control – This is an RNA or DNA that is present
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