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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20°-20°C
- 保质期:
1-3年
- 库存:
999
- 供应商:
亦高生物
- CAS号:
73030-71-4
- 规格:
A2987-10MG

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文献和实验Standard neutral agarose electrophoresis
8. 1 x TAE buffer - 40mM Tris-acetate, 2mM EDTA, pH 8. 10mg/ml ethidium bromide stock solution 10 x Type III loading buffer (50% glycerol, 0.5% bromophenol blue, 0.5% xylene cyanol, this solution should be stored at 4°C) N.B: Extreme caution
PEG Preparation of Plasmid DNA(聚乙二醇制备质粒)
is a modification of the rapid alkaline lysis method of Ish-Horowitz and Burke (1981). You will need 3 basic solutions:- Solution I: 50mM glucose, 25mM Tris.Cl, pH 8, 10mM EDTA Solution II: 0.2M NaOH, 1%(w/v) SDS Solution III: 3M potassium acetate, 2M acetic
PEG Preparation of Plasmid DNA
. The procedure is a modification of the rapid alkaline lysis method of Ish-Horowitz and Burke (1981). You will need 3 basic solutions:- Solution I: 50mM glucose, 25mM Tris.Cl, pH 8, 10mM EDTA Solution II: 0.2M NaOH, 1%(w/v) SDS Solution III: 3M potassium
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