- ￥839
- Sigma
- 美国
- 21639-5ML
- 2026年01月03日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20°-20°C
- 保质期：
1-3年
- 库存：
999
- 供应商：
亦高生物
- CAS号：
124-07-2
- 规格：
21639-5ML
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文献和实验PRODUCTION OF COMPLETELY ES CELL-DERIVED FETUSES BY AGGREGATION WITH TETRAPLOID
of aggregation plate Materials: Dissecting microscope; Sterile tissue culture dishes (Easy Grip 35 x 10 mm, Falcon 3001-3); 1 ml syringe with 26 G 1/2 needle of KSOM medium; light mineral oil (EMBRYO TESTED) (e.g. Sigma: M8410);
will not grow in Schneider's medium from Sigma] 50 ml Fetal Bovine Serum (JRH #12103-78P) - Heat Inactivated (aliquoted in -20) 5 ml 1:100 Penicillin-Streptomycin (Gibco #15070-063) (aliquoted in -20) Sterile filter (0.2 µ) Store at 4o C
Dissociated Retina Immunohistochemistry
Solutions 100X trypsin 10 mg/ml in 1X PBS Sigma #T2271 100X soybean trypsin inhibitor 10 mg/ml in 1X PBS Sigma #T6522 1000X DNaseI 2mg/ml in HBSS Sigma #D4513 Explant Culture
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