- ￥846
- Sigma
- 美国
- 411094-5ML
- 2025年12月29日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20°-20°C
- 保质期：
1-3年
- 库存：
999
- 供应商：
亦高生物
- CAS号：
2351-33-9
- 规格：
411094-5ML
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文献和实验PRODUCTION OF COMPLETELY ES CELL-DERIVED FETUSES BY AGGREGATION WITH TETRAPLOID
of aggregation plate Materials: Dissecting microscope; Sterile tissue culture dishes (Easy Grip 35 x 10 mm, Falcon 3001-3); 1 ml syringe with 26 G 1/2 needle of KSOM medium; light mineral oil (EMBRYO TESTED) (e.g. Sigma: M8410);
will not grow in Schneider's medium from Sigma] 50 ml Fetal Bovine Serum (JRH #12103-78P) - Heat Inactivated (aliquoted in -20) 5 ml 1:100 Penicillin-Streptomycin (Gibco #15070-063) (aliquoted in -20) Sterile filter (0.2 µ) Store at 4o C
-Methylene-bisacrylamide (Kodak 8383) Dissolve in approx. 80 ml of double distilled water and then deionize by stirring with 5 g of Amberlite MB-1 (Sigma MB-1A) for 1 hour at room temperature. Suction filter to remove the Amberlite and adjust to a final
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