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- 文献和实验
- 技术资料
- 保存条件:
-20°-20°C
- 保质期:
1-3年
- 库存:
999
- 供应商:
亦高生物
- CAS号:
103-50-4
- 规格:
33630-250ML

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文献和实验PRODUCTION OF COMPLETELY ES CELL-DERIVED FETUSES BY AGGREGATION WITH TETRAPLOID
of aggregation plate Materials: Dissecting microscope; Sterile tissue culture dishes (Easy Grip 35 x 10 mm, Falcon 3001-3); 1 ml syringe with 26 G 1/2 needle of KSOM medium; light mineral oil (EMBRYO TESTED) (e.g. Sigma: M8410);
实验材料 •BSA(1 mg/ml),0.05 g的BSA(Sigma-Aldrich Ltd.)溶于50ml ddH2O中,每Eppendorf管约加入1ml溶液,并在-20℃下冷冻。 •预染色marker (Bio-Rad) •ProMarker(Wealtec) •两套玻璃板与校正卡,厚玻璃板与U形玻璃板,0.75 mm厚胶条和一个0.75 mm 10齿梳(Wealtec) •浓度为12%的SDS-PAGE分离胶(0.75 mm);2.31 ml ddH
Preparation of DNA Template For Direct Sequencing of Large Insert PAC and BAC Plasmid
) and multi-channel devices from the initial inoculation step to the final cycle sequence reaction. It will yield approximately 200ng of purified plasmid per ml of culture fluid. When using one of the accompanying sequencing methods, one cycle sequence
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